Fig. 4

RT-qPCR and western blotting validation of the expression level of SUCLG1, PCK2, GLDC in OSRC-2 and A498 cell lines. a–c Forty-eight hours after plasmid or vector transfection, qPCR detected the expression level of 3 genes in both OSRC-2 and A498 cell lines. b–f Protein expression was evaluated by Western blot. j–l The values of the band intensity represent the densitometric estimation of each band normalised by β-actin in (b–f, respectively). (*p < 0.05 and **p < 0.01)