Table 1 List of the methylation-specific PCR primer sequence
CG sites | Sequence |
|---|---|
cg11201447 | |
 Forward | AATTTGATATAGTTTTGTGGTTATAGC |
 Reverse | AATTCTATATTTATCCTTCTACAACTTCC |
cg16186435 | |
 Forward | TTTTTAGTTTTTGGGTGGGGAC |
 Reverse | CCACTACTACAATCACTCCATACT |
cg16201808 | |
 Forward | GGCGTTAGAGTGGATATTGC |
 Reverse | ATATACCTTTTACCTTCTACCATAATC |
cg20535723 | |
 Forward | AGTTAGTGGGGAGTGAGGTC |
 Reverse | CCAATCTTACAACTTTCTAAAATAACAAAT |
cg03209812 | |
 Forward | GTTTGTGGTAGAAAAATCGAGTTTAAGT |
 Reverse | TATAATAACGCTTCCCCTCTCCTAA |
cg14743534 | |
 Forward | TAGCGGTGGGTGGGGTCG |
 Reverse | AAACTTCATCACCAAACTCGTAAACAT |
ACTB | |
 Forward | TGGTGATGGAGGAGGTTTAGTAAGT |
 Reverse | AACCAATAAAACCTACTCCTCCCTTAA |