Fig. 4

Co-IP was used to validate the iTRAQ results of proteins interacting with FASN. FASN-specific antibody was used to capture FASN-binding proteins. Normal rabbit IgG and agarose beads were used as a negative control in the hepatoma cell lines HepG2 and SMMC7721. All the co-IP and western blot analyses were performed at least three times. Co-IP co-immunoprecipitation, iTRAQ isobaric tags for relative and absolutely quantitation, FASN fatty acid synthase