Fig. 3

Overexpressed SATB2-AS1 suppresses malignant phenotypes of BC cells. a Expression of SATB2-AS1, miR-155-3p and BRMS1L mRNA in BC cell lines was detected by RT-qPCR after SATB2-AS1 intervention; b protein expression of BRMS1L in and BC cell lines was determined by western blot analysis after SATB2-AS1 intervention; c proliferation of BC cells was determined by MTT assay after SATB2-AS1 intervention; d colony formation ability of BC cells was measured by colony formation assay after SATB2-AS1 intervention; e flow cytometry was used to evaluate the apoptosis of BC cells after SATB2-AS1 intervention; f Transwell assay was employed to measure the migration ability of BC cells after SATB2-AS1 intervention; g Transwell assay was employed to measure the invasion ability of BC cells after SATB2-AS1 intervention; h tumor volume of nude mice that had been injected with BC cells was measured after SATB2-AS1 intervention; i tumor weight of nude mice that had been injected with BC cells was measured after SATB2-AS1 intervention. The measurement data conforming to the normal distribution were expressed as mean ± standard deviation, one-way ANOVA was used for comparisons among multiple groups and Tukey’s post hoc test was used for pairwise comparisons after one-way ANOVA