Fig. 5

SATB2-AS1 serves as a ceRNA to sponge miR-155-3p, and BRMS1L is targeted by miR-155-3p. a The predicted binding sites of SATB2-AS1 and miR-155-3p; b regulatory relation between SATB2-AS1 and miR-155-3p in MDA-MB-231 and MCF-7 cells was assessed by dual luciferase reporter gene assay; c regulatory relation between SATB2-AS1 and miR-155-3p in MDA-MB-231 and MCF-7 cells was confirmed by RNA pull-down assay; d predicted binding sites of miR-155-3p and BRMS1L 3′-UTR; e target relation between miR-155-3p and BRMS1L was confirmed by dual luciferase reporter gene assay; the measurement data conforming to the normal distribution were expressed as mean ± standard deviation, the t-test was performed for comparisons between two groups, one-way ANOVA was used for comparisons among multiple groups and Tukey’s post hoc test was used for pairwise comparisons after one-way ANOVA