Fig. 1

Linc00473 expression level was clearly up-regulated in CCA tissues and cells. a Data mining of differentially expressed lncRNAs expression in TCGA dataset. b Linc00473 expression was measured by qRT-PCR in CCA tissues and matched adjacent normal tissues (Normal tissues). c, d The relative linc00473 up-regulation was significantly associated with advanced TNM stage and lymph node invasion. e The Correlation between linc00473 expression and the overall survival of CCA patients. f Linc00473 expression was up-regulated in five CCA cell lines compare with HIBEC. g The linc00473 expression was measured in the nucleus and cytoplasm of CCA cells (CCLP1, HCCC-9810). U6 (nuclear retained) and GAPDH (exported to cytoplasm) were served as controls. h CCLP1 and HCCC-9810 cells were transfected with si-NC, si-linc00473 for 24 h, linc00473 expression was using qRT-PCR. i Linc00473 expression was significantly increased after transfected with pcDNA3.1-NC/pcDNA3.1-linc00473. The error bars indicate the mean ± SD, and each experiment was repeated at least three times. *p < 0.05, **p < 0.01, ***p < 0.001