Fig. 4

Linc00473 acted as miR-506 sponge in CCA cells. a, b Heatmaps were drawn to show the differentially expressed miRNAs in CCLP1 and HCCC-9810 cells with depletion of linc00473. c Venn diagram shows the overlap between the up-regulated miRNAs in CCLP1 and the up-regulated ones in HCCC-9810. d CCLP1 cells were transfected with si-NC, si-linc00473-1 or si-linc00473-2 for 24 h, expression of miRNAs was detected. e The differential expression of miR-506 in CCA tissues and adjacent normal bile duct tissues was analyzed by qRT-PCR. f Pearson’s correlation curve revealed the negative relevance between linc00473 and miR-506 expression. g The miR-506 expression level was investigated in CCA cells (CCLP1, HCCC-9810) and HIBEC. h Sequence alignment of miR-506 with the putative binding sites within the wild-type regions of linc00473. i, j CCLP1 and HCCC-9810 cells were co-transfected with miR-506 mimics and linc00473 WT vector linc00473 MUT vector for 48 h, the luciferase activity was measured. k, l The expression of linc00473 and miR-506 in the Ago2-containing beads in CCLP1 and HCCC-9810, compared with the beads harboring control IgG. The error bars indicate the mean ± SD, and each experiment was repeated at least three times. **p < 0.01, ***p < 0 .001