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Fig. 5 | Cancer Cell International

Fig. 5

From: Linc00473 potentiates cholangiocarcinoma progression by modulation of DDX5 expression via miR-506 regulation

Fig. 5

DDX5 was a novel target of miR-506, and linc00473 positively regulated DDX5 through binding with miR-506. a The 3′-UTR of DDX-5 harbored miR-506 cognate site. b Representative images of DDX5 immunohistochemistry (IHC) staining in normal bile duct tissues and CCA tissues. c The expression correlation between linc00473 and DDX5 mRNA in CCA tissues by Pearson’s correlation curve. d, e CCLP1 and HCCC-9810 were co-transfected with miR-506 mimics and wild-type or mutant DDX-5 3′-UTR for 48 h, the luciferase activity was determined. f CCA cells (CCLP1 and HCCC-9810) were transfected with miRNA NC or miR-506 mimics for 48 h, protein level of DDX-5 was determined. g, h Western blot results indicated that linc00473 depletion significantly decreased DDX5 expression, besides linc00473 overexpression significantly increased DDX5 expression as well. Results shown are the mean ± SD (*p < 0.05, **p < 0.01) of triplicate determination from three independent experiments

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