Fig. 5

VAMP8 is the target of miR345-3p and regulated by LINC01426. a The binding site of VAMP8 and miR345-3p was predicted by StarBase. b qRT-PCR determine the expression of VAMP8 in GBM cell lines. c, d The VAMP8 expression level was positively correlated with LINC01426 in HS683 and U251 (c) or SW1783 (d) stable cell lines. e, f RIP assays using the Ago2 antibody to detect the enrichment of LINC01426, miR345-3p and VAMP8 in U251 (e) and SW1783 (f). g Luciferase reporter assays demonstrated the interaction between LINC01426, miR345-3p in HEK-293T cell transfected with VAMP8 wild type or mutant reporter plasmids. In B-G, the data are represented as mean ± SD of three times