Fig. 1

The identification and characteristics of circRNA. a By analyzing GSE102686 data series, we identified 21 differentially expressed circRNAs in cervical cancer, and the top five most significantly overexpressed circRNAs were presented here. The criteria for differential expression was adj. P < 0.05 and log|FC| ≥ 1.5. b–f The qRT-PCR results for the top five most significantly upregulated circRNAs in our collected tumorous cervical tissues and healthy adjacent tissues. N = 30. g qRT-PCR results for the expression of circ_0084927 in selected cell lines. Apart from the HcerEpic cell line, others are cervical cancer cell lines. *P < 0.05, **P < 0.01, compared with the HcerEpic cell line. h The structure of circ_0084927 was illustrated. It consists of three exons 7–9 from ESRP1 host gene. i The RNase R + tolerance feature of circ_0084927 in Hela and C-33A cell lines. **P < 0.01, compared with the control group without RNase R treatment. j The localization of circ_0084927 in Hela and C-33A cell lines using a cell fractionation method. **P < 0.01, compared with the level of circ_0084927 or linear ESRP1 mRNA in cytoplasm