Fig. 5

miR-1179 directly inhibited CDK2 mRNA expression by binding to its 3′UTR. a The potential binding site between miR-1179 and CDK2 was predicted by TargetScan Human 7.2. b The potential binding between miR-1179 and the 3′UTR of CDK2 gene was validated by the luciferase reporter gene assay. CDK2 mutant or CDK2 wild-type plasmids containing the fluorescence group and miR-1179 were co-transfected into HeLa and C-33A cells. **P < 0.01, compared with the NC group. NC = negative control. c RNA pull-down assay was used to validate the interaction between CDK2 mRNA and miR-1179. Bio = biotin-labelled. *P < 0.05, compared with the bio-NC group. d CDK2 expression in CC tissues and normal tissues was detected by qRT-PCR. e The correlation between miR-1179 and CDK2 expression was identified by spearman correlation analysis (b–e). In the three experiments, data were formatted in the form of mean ± SD