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Fig. 3 | Cancer Cell International

Fig. 3

From: EO771, the first luminal B mammary cancer cell line from C57BL/6 mice

Fig. 3

Estradiol and leptin do not influence EO771 cell phenotype and viability. a, b ERα, ERβ and PR protein levels was assayed by western blot on EO771 cells (representative of 3 experiments) and normalized to the actin protein levels in presence of estradiol (Low: 1.5 ng/mL; High: 225 ng/mL) or leptin (Low: 10 ng/mL; High: 100 ng/mL) in untreated conditions (DMSO) (a) or with tamoxifen (b). c, d Cell viability was measured by fluorescence using resazurin solution. EO771 cells were cultured in the presence of estradiol, leptin or vehicle (c) and EO771 cells were treated with increasing tamoxifen concentration (d). The untreated conditions corresponded to a viability of 100%. The tamoxifen IC50 corresponded to the tamoxifen concentration inducing 50% of EO771 viability (e)

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