Fig. 2

Circ-ITCH overexpression suppressed proliferation, invasion and glycolysis, and promoted cell apoptosis of ovarian cancer cells. a, b After RNase R treatment, linear-ITCH and circular-ITCH expression levels in A2780 and OVCAR3 cells were measured by qRT-PCR. c, d Relative expression of circ-ITCH in Nuclear and cytoplasm of A2780 and OVCAR3 cells was determined by qRT-PCR. e Circ-ITCH expression in A2780 and OVCAR3 cells transfected with pcDNA or circ-ITCH (pcDNA-circ-ITCH) was examined by qRT-PCR. f, g The proliferation ability of A2780 and OVCAR3 cells transfected with pcDNA or circ-ITCH was examined by CCK-8 assay. h The invasion of A2780 and OVCAR3 cells transfected with pcDNA or circ-ITCH was measured by Transwell assay with Matrigel. i–k Glucose consumption, lactate production and ATP level in A2780 and OVCAR3 cells transfected with pcDNA or circ-ITCH were detected using glucose, lactate and ATP assay kits, respectively. l The apoptosis of A2780 and OVCAR3 cells was detected by Flow cytometry. **P < 0.01, ***P < 0.001