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Fig. 8 | Cancer Cell International

Fig. 8

From: Circular RNA ITCH suppresses proliferation, invasion, and glycolysis of ovarian cancer cells by up-regulating CDH1 via sponging miR-106a

Fig. 8

CDHI knockdown reversed the suppression action of circ-ITCH on ovarian cancer cell progression. a Western blot assay was applied to detect CDH1 protein level in A2780 and OVCAR3 cells transfected with pcDNA, circ-ITCH, circ-ITCH + si-con, or circ-ITCH + si-CDH1. b CCK-8 assay was performed to assess proliferation in transfected A2780 and OVCAR3 cells. c Transwell assay with Matrigel was used to measure invasion in A2780 and OVCAR3 cells. d, f Glucose, lactate and ATP assay kits were employed to examine glucose consumption, lactate production and ATP level in transfected A2780 and OVCAR3 cells, respectively. g Flow cytometry assay was conducted to assess apoptosis rate in transfected A2780 and OVCAR3 cells. **P < 0.01, ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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