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Fig. 2 | Cancer Cell International

Fig. 2

From: Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7

Fig. 2

Influence of hsa_circ_0000735 knockdown on the sensitivity of DTX-resistant PCa cells to DTX. a The illustration exhibited siRNAs (si-circ-1 and si-circ-2) that target the junction of hsa_circ_0000735. b, c QRT-PCR results for P2RX1 mRNA and hsa_circ_0000735 in PC-3/DTX and DU145/DTX cells transfected with si-NC, si-circ-1, or si-circ-2. d, e The viability of PC-3/DTX and DU145/DTX cells transfected with si-NC or si-circ-1 in the presence of DTX at the different concentrations (5, 10, 20, 40, 80, or 160 nM) for 48 h was determined through CCK-8 assay. f–k The viability, colony formation, cell cycle progression, and apoptosis of PC-3/DTX and DU145/DTX cells transfected with si-NC or si-circ-1 under DTX (10 nM) treatment were assessed with CCK-8, cell colony formation, or flow cytometry assays. l, m The levels of MDR1, cyclinD1, and bcl-2 in PC-3/DTX and DU145/DTX cells transfected with si-NC or si-circ-1 under DTX (10 nM) treatment were assessed via western blotting. *P < 0.05 and ***P < 0.001

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