Fig. 3

Hsa_circ_0000735 served as a sponge for miR-7 in DTX-resistant PCa cells. a, b QRT-PCR was conducted to detect the levels of hsa_circ_0000735 in cytoplasm and nucleus of PC-3/DTX and DU145/DTX cells. c, d RIP assay was carried for the assessment of hsa_circ_0000735 level in PC-3/DTX and DU145/DTX cells transfected with si-circ-1 or si-NC. e Schematic illustration the overlap of the target miRNAs of hsa_circ_0000735 predicted by circBank and circinteractome databases. f, g RNA pull-down assay was carried out by using hsa_circ_0000735 probe to pull down miR-1182, miR-331-3p, miR-583, and miR-7. h The binding sites between hsa_circ_0000735 and miR-7 were exhibited. i Schematic diagram of the luciferase reporters, which contained the WT-hsa_circ_0000735, MUT1-hsa_circ_0000735, MUT2-hsa_circ_0000735, or MUT1 + 2-hsa_circ_0000735 sequences. j, k Dual-luciferase reporter assay was conducted to analyze the luciferase intensity of the luciferase reporters in PC-3/DTX and DU145/DTX cells transfected with miR-NC or miR-7. l The expression of miR-7 in PC-3/DTX and DU145/DTX cells transfected with si-circ-1 or si-NC was analyzed via qRT-PCR. *P < 0.05, **P < 0.01, and ***P < 0.001