Fig. 4

Effects of miR-7 mimic on the sensitivity of DTX-resistant PCa cells to DTX. a QRT-PCR was conducted to examine the expression of miR-7 in 23 DTX-sensitive PCa tissues, 27 DTX-resistant PCa tissues, and 50 adjacent normal tissues. b QRT-PCR was performed to analyze the expression of miR-7 in PC-3, DU145, PC-3/DTX, DU145/DTX, and RWPE-1 cells. c, d CCK-8 assay was carried out to evaluate the sensitivity and IC50 value of PC-3/DTX and DU145/DTX after miR-7 or miR-NC transfection. e–j The viability, cell cycle progression, and apoptosis of PC-3/DTX and DU145/DTX cells transfected with miR-7 or miR-NC under DTX (10 nM) treatment were assessed with CCK-8, cell colony formation, or flow cytometry assays. k, l Western blotting was performed to examine the levels of MDR1, cyclinD1, and bcl-2 in PC-3/DTX and DU145/DTX cells transfected with miR-7 or miR-NC under DTX (10 nM) treatment. *P < 0.05, **P < 0.01, and ***P < 0.001