Fig. 5

Hsa_circ_0000735 regulated the resistance of PCa to DTX through miR-7. a The expression of miR-7 in PC-3/DTX and DU145/DTX cells transfected with anti-miR-NC or anti-NC was evaluated by qRT-PCR. b–l PC-3/DTX and DU145/DTX cells were transfected with si-NC + anti-NC, si-circ-1 + anti-NC, or si-circ-1 + anti-miR-7, respectively. b–d The DTX sensitivity and IC50 value of PC-3/DTX and DU145/DTX cells were assessed with CCK-8 assay. e–j The viability, colony formation, cell cycle progression, and apoptosis of PC-3/DTX and DU145/DTX cells after DTX (10 nM) treatment were determined via CCK-8, cell colony formation, or flow cytometry assays. k, l The levels of MDR1, cyclinD1, and bcl-2 of PC-3/DTX and DU145/DTX cells treated with DTX (10 nM) were detected by western blotting. *P < 0.05, **P < 0.01, and ***P < 0.001