Fig. 2

Circ_0000190 inhibited cancer cell growth. a Expression level of circ_0000190 in normal gastric cells (GES) and gastric cancer cells (MGC-803, SGC-7901, BGC-823 and AGS) was detected by RT-PCR, *P < 0.05, ***P < 0.001 compared with GEC cells. b Location of circ_0000190 in BGC-823 and MGC-803 cells was examined by FISH. The empty vectors and those inserted with circ_0000190 were individually transfected into BGC-823 and MGC-803 cells. c The expressions of circ_0000190 in the primary or transfected BGC-823 and MGC803 cells was examined by RT-PCR, ***P < 0.001 compared with the control or NC cells. d Cell viability were examined by CCK-8 assay at 24 h, 48 h and 72 h after culture, ***P < 0.001 compared with the control or NC cells at the same condition. Cell apoptosis of the primary and transfected BGC-823 and MGC-803 cells was examined by flow cytometry (e) and Hoechst staining (f), the cell proliferation was detected by EdU (g) and colony formation assay (h), cell invasion and migration were examined by transwell (i) and scratch (j) assay, respectively