Fig. 6

Overexpression of CTNND1 relieved the inhibitory effects of miR-329-3p on cell proliferation, migration, invasion and suppressed apoptosis. SW480 and LS1034 cells were transfected with control RNA or miR-329-3p together with plasmid expressing CTNND1 or empty vector. a The expression of CTNND1 in SW480 and LS1034 cells was tested by western blot. b, c SW480 and LS1034 cell proliferation were presented by MTT assay at 24 h, 48 h, and 72 h. d Flow cytometry was used to evaluate apoptosis of SW480 and LS1034 cells. e, f Cell migration and invasion in SW480 and LS1034 was detected by transwell assay. g, h The protein levels of EMT markers (E-cadherin, MMP9), CyclinD1, apoptosis-associated proteins (Bcl-2, Cleaved-casp-3) in SW480 and LS1034 cells were measured by western blot assays. *P < 0.05, vs. control group