Fig. 7

KCNQ1OT1 up-regulated CTNND1 expression via sponging miR-329-3p. a The correlation of KCNQ1OT1 and CTNND1 in CRC tissues was examined by spearman’s correlation analysis (P < 0.0001, r = − 0.6758). b, c SW480 and LS1034 cells were co-transfected with si-con/si-KCNQ1OT1 and si-KCNQ1OT1 + anti-con/si-KCNQ1OT1 + anti-miR-329-3p for 24 h, and then western blot was performed to check the level of CTNND1. d, e MTT assay was used to determine the viability of SW480 and LS1034 cells transfected with si-KCNQ1OT1 or si-KCNQ1OT1 + pcDNA-CTNND1. f And the cell apoptosis rates were measured by flow cytometry in SW480 and LS1034 cells transfected with si-KCNQ1OT1 or si-KCNQ1OT1 + pcDNA-CTNND1. g, h Transwell assay was conducted to evaluate the migratory and invasive ability of SW480 and LS1034 cells transfected with si-KCNQ1OT1 or si-KCNQ1OT1 + pcDNA-CTNND1. i, j The expression of EMT markers (E-cadherin, MMP9), CyclinD1, apoptosis-associated proteins (Bcl-2, Cleaved-casp-3) were evaluated by western blot in SW480 and LS1034 cells transfected with si-KCNQ1OT1 or si-KCNQ1OT1 + pcDNA-CTNND1. *P < 0.05, vs. control group