Fig. 2

CTBP1-AS2 acts as a miR-3163 sponge in CC. a, b Subcellular fractionation and FISH assays were performed to determine the subcellular location of CTBP1-AS2. c qRT-PCR analysis was performed to evaluate miR-3163 expression after knockdown of CTBP1-AS2. d qRT-PCR analysis was conducted to examine the expression of miR-3163 in CC cell lines and normal cell lines. e Putative binding of miR-3163 and CTBP1-AS2 was predicted by starBase tool. f Dual luciferase reporter assays were performed in HeLa and SiHa cell lines. g RNA pull down was performed to determine the interaction between miR-3163 and CTBP1-AS2. h The transfection efficiency of miR-3163 inhibitor was determined by qRT-PCR. i EdU was performed to determine cell proliferation with inhibited miR-3163 to rescue down-regulated CTBP1-AS2. j TUNEL was conducted to examine cell apoptosis with inhibited miR-3163 to rescue down-regulated CTBP1-AS2. k, l Transwell assays were used to measure cell migration and invasion with inhibited miR-3163 to rescue CTBP1-AS2. ^**P < 0.01