Fig. 3

MiR-3163 targets ZNF217 in CC. a qRT-PCR was performed to study the expression of target genes after overexpressing miR-3163. b qRT-PCR analysis was conducted to examine the ZNF217 expression in CC cell lines and normal cell lines. c Potential binding sites between ZNF217 and miR-3163 were predicted by starBase website. d Luciferase reporter assay was performed to detect the interaction between ZNF217 and miR-3163 in HEK-293T. e RIP demonstrated that miR-3163, CTBP1-AS2 and ZNF217 co-existed in RISC. f The transfection efficiency of sh-ZNF217 plasmids was ensured by qRT-PCR. g–k. Functional experiments was carried to study the role of ZNF217 in CC cells. ^*P < 0.05, ^**P < 0.01