Fig. 4

ZNF217 overexpression reverses the anti-tumor effects of CTBP1-AS2 knockdown. a qRT-PCR analysis was used to determine the expression of ZNF217 after transfecting with pcDNA3.1 and pcDNA3.1/ZNF217 plasmids. b, c qRT-PCR and western blot were performed to detect the expression of ZNF217 mRNA and protein. d CCK8 assay was used to evaluate cell vitality. e TUNEL assay was conducted to evaluate cell apoptosis. f, g Transwell assays were performed to measure cell migration and invasion ability respectively. h Western blot was performed to measure the expression of apoptosis-related proteins. ^*P < 0.05, ^**P < 0.01