Fig. 4

MIAT could serve as a miR-149-5p sponge, and miR-149-5p counters the oncogenic effects of MIAT in NSCLC cells. a Schematic representation of the potential binding sites of miR-149-5p and MIAT. b The luciferase activities were assessed after wild-type or mutant MIAT luciferase reporter vector was co-transfected with miR-149-5p mimic or negative control mimic into A549 and H1299 cells. c The expression levels of miR-149-5p in lung cancer cells and normal lung cells were measured by qRT-PCR. d Pearson correlation analysis showed that miR-149-5p expression was negatively correlated with that of MIAT. e, g, i CCK-8, Colony formation and transwell assays revealed that silencing of MIAT inhibited A549 and H1299 cell proliferation, and these inhibition effects were attenuated by miR-149-5p inhibitor. f, h, j Overexpression of MIAT promoted A549 and H1299 cell proliferation, and these inhibition effects were reversed by miR-149-5p mimic. *p < 0.05, ***p < 0.001