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Fig. 2 | Cancer Cell International

Fig. 2

From: RETRACTED ARTICLE: Long non-coding RNA LBX2-AS1 enhances glioma proliferation through downregulating microRNA-491-5p

Fig. 2

LBX2-AS1 expression was associated with miR-491-5p levels in glioma. a RT-PCR detection of LBX2-AS1 expression in a panel of glioma cell lines U87MG, U251MG, A172 and normal human astrocyte (NHA). b Flow chart of screen for target miRNAs of LBX2-AS1 in glioma. c The association between LBX2-AS1 and miR-491-5p expression in TCGA-LGG project was analyzed by Pearson correlation analysis. d RT-PCR detection of miR-491-5p expression in normal brains and gliomas. e Comparison of miR-491-5p expression in normal brain, grade II glioma, grade III glioma and grade IV glioma. f The association between LBX2-AS1 and miR-491-5p expression in collected glioma samples was analyzed by Pearson correlation analysis. g The expression of miR-491-5p was detected in U87MG and U251MG cells transfected with miR-NC or miR-491-5p mimic. In U87MG (h) and U251MG (i) cells, the luciferase activity of pmirGLO (empty vector as the control group) and pmirGLO-LBX2-AS1 was detected. J. RIP assay was performed with IgG and Ago2 antibody, followed by RT-PCR detection of LBX2-AS1 and miR-491-5p levels. *, p < 0.05; ***, p < 0.001

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