Fig. 5

LBX2-AS1 regulated TRIM28 expression via sponging miR-491-5p. a The expression of miR-491-5p was detected in U87MG and U251MG cells transfected with miR-NC or miR-491-5p inhibitor. b The expression of TRIM28 mRNA was detected in U87MG and U251MG cells transfected with miR-NC or miR-491-5p inhibitor in combination with si-Control or si-LBX2-AS1-1 by RT-PCR. c The expression of TRIM28 protein was detected in U87MG cells transfected with miR-NC or miR-491-5p inhibitor in combination with si-Control or si-LBX2-AS1-1 by western blotting. d Sequence alignment of LBX2-AS1 TRIM28 3′UTR WT, TRIM28 3′UTR Mut and miR-491-5p. e Luciferase activity was detected in U87MG cells transfected with TRIM28 3′UTR WT or TRIM28 3′UTR Mut in combination with si-Control or si-LBX2-AS1-1 and miR-NC or miR-491-5p inhibitor. **, p < 0.01; ***, p < 0.001