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Fig. 1 | Cancer Cell International

Fig. 1

From: RACK1 promotes miR-302b/c/d-3p expression and inhibits CCNO expression to induce cell apoptosis in cervical squamous cell carcinoma

Fig. 1

CCNO is up-regulated in CSCC, and down-regulated CCNO induces apoptosis of CSCC cells. a The expression of CCNO in CSCC and endocervical adenocarcinoma from GEPIA database; b, c CCNO expression in CSCC (N = 46) and normal cervical epithelial tissues (N = 30) detected by RT-qPCR and immunohistochemistry (× 400); CCNO mRNA level in CSCC cell line (CasKi) and normal cell line (H8) detected by RT-qPCR; e The protein levels of CCNO, Bcl-2, Cyclin D1, cleaved PARP, cleaved caspase 3 in CSCC cell line (CasKi) and normal cell line (H8) detected by Western blot analysis, with the protein bands assessed; f The protein levels of CCNO, Bcl-2, Cyclin D1, cleaved PARP and cleaved caspase 3 in CasKi cells with CCNO silencing detected by Western blot analysis, with the protein bands assessed; g Cell cycle entry in CSCC cell line (CasKi) detected by flow cytometry; h Cell proliferation detected by EdU assay (×200); i Cell apoptosis detected by TUNEL assay (×200). *p < 0.05 vs. normal cervical epithelial tissues, H8 cell line or cells treated si-NC. The values in this figure were all measurement data and expressed as mean ± standard deviation. Independent sample t test was used for the data comparison between two groups. The experiment was repeated 3 times independently

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