Fig. 2

miR-302b/c/d-3p targets CCNO and inhibits its expression. a The comparison of upstream miRNA of CCNO predicted by RNA22, miRwalk, TargetScan, and mirDIP; b The binding site between miR-302b/c/d-3p and CCNO-3′-UTR; c–e Luciferase activity in cells after transfection with miR-302b/c/d-3p and WT-CCNO or miR-302b/c/d-3p and MUT-CCNO detected by dual luciferase reporter assay; f The expression of miR-302b/c/d-3p and CCNO in cells detected by RT-qPCR; g CCNO protein expression detected by Western blot analysis, with the protein bands assessed; h–j Correlation analysis between CCNO expression and miR-302b/c/d-3p expression in CasKi cells. *p < 0.05 vs. the cells treated with NC mimic. The values in the figure were measurement data, and expressed as mean ± standard deviation. Independent sample t test was used for data comparison between two groups. One-way ANOVA was used for data comparison among multiple groups, and followed by Tukey’s post hoc test. Pearson’s correlation coefficient was used for correlation analysis between indicators. The experiment was repeated 3 times independently