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Fig. 3 | Cancer Cell International

Fig. 3

From: RACK1 promotes miR-302b/c/d-3p expression and inhibits CCNO expression to induce cell apoptosis in cervical squamous cell carcinoma

Fig. 3

miR-302b-3p, miR-302c -3p or miR-302d-3p inhibits proliferation while inducing apoptosis of CSCC cells by targeting CCNO. ac The expression of miR-302b/c/d-3p in CSCC (N = 46) and normal cervical tissues (N = 30) detected by RT-qPCR; d The expression of miR-302b/c/d-3p in CSCC cell line (CasKi) and normal cell line (H8) detected by RT-qPCR; e The protein levels of CCNO, Bcl-2, Cyclin D1, cleaved PARP, cleaved caspase 3 in CSCC cell line (CasKi) detected by Western blot analysis, with protein bands assessed; f The cell cycle entry in CSCC cell line (CasKi) detected by flow cytometry; g The cell proliferation detected by EdU assay (×200); h The cell apoptosis detected by TUNEL assay (×200). *p < 0.05 vs. normal cervical tissues, H8 cell line or cells treated with NC mimic. The values in the figure were all measurement data and expressed as mean ± standard deviation. Independent sample t test was used for data comparison between two groups, and one-way ANOVA with Tukey’s post hoc test was used for data comparison among multiple groups. The experiment was repeated 3 times independently

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