Fig. 4

MiR-361-3p interacts with SOX4 and negatively regulates its expression in the cytoplasm in CC cells. a The potential binding sequence between SOX4 and miR-361-3p was listed. b, c Luciferase reporter assay was performed in SiHa and HeLa cells the co-transfected with SOX4 3′UTR-WT or SOX4 3′UTR-WT-MUT and miR-361-3p mimic or mimic-NC. d, f The mRNA expression of SOX4 was detected using qRT-PCR in CC tissues and cell lines. e The correlation between SOX4 and miR-361-3p expression was analyzed in CC patients. g Western blot was used to measure the level of SOX4 protein in SiHa, HeLa and End1/E6E7 cells. h, i The mRNA and protein expression of SOX4 was qualified using qRT-PCR or western blot in SiHa and HeLa cells transfected with miR-361-3p mimic or mimic-NC. *P < 0.05