Fig. 5

CircUBAP2 deletion inhibits the CC cell progression in vitro via severing as a ceRNA of miR-361-3p to regulate SOX4. SiHa and HeLa cells were transfected with sh-NC or sh-circUBAP2 or sh-circUBAP2 and miR-361-3p inhibitor or sh-circUBAP2 and SOX4 pcDNA. a The mRNA and protein expression of SOX4 in SiHa and HeLa cells was detected using qRT-PCR or western blot, respectively. b, c The proliferation ability was assessed using MTT assay in SiHa and HeLa cells. d, e The apoptosis rates were analyzed by Flow Cytometry in SiHa and HeLa cells. f, g The protein levels of cleaved caspase-3, Bcl-2 and Bax in SiHa and HeLa cells were examined by western blot. h, i The migration and invasion ability of SiHa and HeLa cells was examined by transwell assay. j, k The levels of E-cadherin, N-cadherin and Vimentin were determined using western blot. *P < 0.05