Fig. 6

LRIG2 was a direct target of miR-182. a The binding site of LRIG2 and miR-182 was predicted by TargetScan. b, c The relative luciferase activity in EC cells was detected by dual-luciferase reporter assay. d The mRNA expression of LRIG2 in EC tissues was examined by RT-qPCR. e Western blot was used to detect the protein expression of LRIG2 in EC tissues. f The negative relationship between miR-182 and LRIG2 was discovered in EC tissues (r = − 0.3122, P < 0.01). g The positive relationship between circ_0001776 and LRIG2 was observed in EC tissues (r = 0.5728, P < 0.01). h LRIG2 expression was determined after EC cells transfected with anti-NC or anti-miR-182. i The protein expression of LRIG2 in EC cells was detected by western blot. j LRIG2 expression in EC cells transfected with anti-NC or anti-miR-182 was measured by western blot. k, l LRIG2 expression in EC cells transfected with vector, circ_0001776, circ_0001776 + miR-NC or circ_0001776 + miR-182 was examined by western blot. **P < 0.01