Fig. 6

Tumorsphere formation of WHO grade II atypical meningioma cell line. a Representative image of primary tumorsphere from atypical meningioma cancer cell line under treatment with EGF and FGF2. Scale bars = 100 µm. b Number of primary, secondary, and tertiary spheres obtained from atypical meningioma cancer cell line per 10,000 viable cells plated. The number of neurospheres was determined 10 days after plating. The value represents mean ± SEM. Data were analyzed by one-way ANOVA. Asterisks indicate statistical significance (****p < 0.0001). c Immunophenotypic characterization by flow cytometry assay showing the expression of dual markers CD44/CD133 in primary atypical meningioma cancer cell line. d Representative image of sorted CD133⁺ or CD133⁻ cell population from CD44⁺ atypical meningioma cancer cell line. Scale bar = 100 µm. e Population doubling time of sorted CD133⁺ or CD133⁻ cell population from CD44⁺ atypical meningioma cancer cell line