Fig. 4

Modulating miR-203 expression in EC cell lines impact Snail1 protein stability. a Representative blots from three experiments showing steady expression of Snail1, USP26 and E-cadherin in the normal esophageal cell line HET-1A and the EC cell lines Kyse150 and TE-1 transfected with either control or MIR203A/5P mimic. GAPDH. Loading control. b TE-1 cells transfected with control or MIR203A/5P mimic were treated with 50 µM cycloheximide (CHX) for up to 8 h and then lysates were probed for Snail1 and GAPDH protein expression. Shown are representative blots from three experiments. c Blots in b were analyzed using NIH ImageJ algorithm and half-life of Snail1 protein calculated