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Fig. 6 | Cancer Cell International

Fig. 6

From: Targeting deubiquitinating enzyme USP26 by microRNA-203 regulates Snail1’s pro-metastatic functions in esophageal cancer

Fig. 6

Suppression of miR-203-mediated regulation of USP26 is required for metastatic progression of EC cells. Xenograft model of experimental metastasis was established by lateral tail vein injection of parental Kyse150 cells or Kyse150 cells stably overexpressing USP26 into BALB/c nude mice (n = 10 and 5 mice, respectively). The mice injected with parental Kyse150 cells were randomly divided into two groups – control mimic (n = 05) (left panels), and MIR203A/5P mimic (n = 05) (middle panels), where the mice injected with Kyse150 cells overexpressing USP26 received MIR203A/5P mimic (right panels). The incidence of metastasis was measured by luciferin injection and bioluminescence imaging. Shown are representative animals from each group after 6 weeks (a). Mice were euthanized at the end of 6 weeks and the lungs from each group of experimental animals were surgically excised, fixed overnight in 10% buffered formalin. Representative images of hematoxylin and eosin (H and E) staining of the lungs are shown (b). Black arrows indicate micrometastasis. Scale bar, 100 µm. c Representative images of IHC staining for USP26 protein in each experimental group. Scale bar, 30 µm

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