Fig. 2
From: USP18 directly regulates Snail1 protein through ubiquitination pathway in colorectal cancer
USP18 promoted colorectal cancer cell proliferation in vitro. a, b Western blot and qRT-PCR analysis of the USP18 protein and mRNA levels in normal colon epithelial cell lines and four common colorectal cancer cells. c, d Western blot and qRT-PCR were used to select sequences with better knockdown efficiency. e, f Western blot and qRT-PCR were used to identify the effects of USP18 knockdown treatment in SW480 cells and USP18 overexpression treatment in DLD1 cells. g, j CCK8 and edu experiment was employed to study the cell proliferation of USP18 knocked-down SW480 cells and USP18 over-expressed DLD1 cells. *P < 0.05, **P < 0.01, ***P < 0.001