Fig. 6
From: USP18 directly regulates Snail1 protein through ubiquitination pathway in colorectal cancer
Snail1 could effectively reverse the influence of USP18 on cell proliferation, migration, invasion, and EMT of CRC cells. a, b Western blot and qRT-PCR analysis of the optimal interference sequence and overexpression sequence of Snail 1 siRNA. c, d CCK8 analysis of the USP18 knocked-down SW480 cells and USP18 over-expressed DLD1 cells with Snail1 siRNA and overexpression plasmid treatments. e, f Scratch experiment analysis of the USP18 over-expressed DLD1 cells and USP18 knocked-down SW480 cells with Snail1 siRNA and overexpression plasmid treatments. g, h Three common chemotherapeutics (fluorouracial, doxorubicin and cisplatin) for cell survival rate analysis of the USP18 knocked-down SW480 cells and USP18 over-expressed DLD1 cells with Snail1 siRNA and overexpression plasmids treatments. *P < 0.05, **P < 0.01, ***P < 0.001