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Fig. 6 | Cancer Cell International

Fig. 6

From: HOXA-AS2 promotes type I endometrial carcinoma via miRNA-302c-3p-mediated regulation of ZFX

Fig. 6

YKL-40 promotes the development of type I endometrial cancer cells and is regulated by ZFX in vitro. a Cell proliferation of YKL-40-modified Ishikawa cells was examined by CCK-8 assay. b Cell cycle phases of YKL-40-modified Ishikawa cells were examined by flow cytometry. c Fractions of apoptotic YKL-40-modified Ishikawa cells were examined by flow cytometry. d Invasion of miR-302c-3p-modified cells was examined by Transwell invasion assays. e, f YKL-40 mRNA and protein levels in ZFX-modified Ishikawa cells were measured by qRT-PCR and western blotting. Data are the mean ± SD from independent experiments. **P < 0.01, ***P < 0.001. g Proposed negative feedback loop mechanism of the HOXA-AS2-miR-302c-3p/ZFX/YKL-40 axis in Ishikawa cells. HOXA-AS2 regulate the biological behavior of the type I endometrium by negatively regulating miR-302c-3p expression. MiR-302c-3p acts as a tumor suppressor in endometrial cancer, low expression of miR-302c-3p promotes the expression of transcription factor ZFX, and high expression of ZFX promotes the expression of hydrolase YKL-40, which in turn promotes malignant behavior of type I endometrium

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