Fig. 3

MiR-326 acted as a target for hsa_circ_0000517 in HCC. a Both Starbase and Circular RNA Interactome databases displayed that miR-1296-5p, miR-326, and miR-330-5p might be potential targets for hsa_circ_0000517. b The binding sites of hsa_circ_0000517 in miR-326 were showed. c, d QRT-PCR was employed to detect miR-326 expression in HCC tissues and cells. e The correlation between hsa_circ_0000517 and miR-326 in HCC tissues was revealed via Pearson’s correlation analysis. f Effect of hsa_circ_0000517 on miR-326 expression of HCCLM3 and Huh7 cells was determined by qRT-PCR. g The luciferase intensity in HCCLM3 and Huh7 cells co-transfected luciferase reporters containing hsa_circ_0000517-wt or hsa_circ_0000517-mut and miR-326 or NC was determined through dual-luciferase reporter assay. h The relationship between hsa_circ_0000517 and miR-326 was further verified via RIP assay. *P < 0.05