Fig. 4

Hsa_circ_0000517 played its role in HCC via miR-326. a The si-NC, si-hsa_circ_0000517#1, si-hsa_circ_0000517#1 + anti-NC, or si-hsa_circ_0000517#1 + anti-miR-326 was transfected into HCCLM3 and Huh7 cells, respectively. a The expression of miR-326 in HCCLM3 and Huh7 cells was analyzed through qRT-PCR. b–f The proliferation, colony formation, cell cycle, migration, and invasion of HCCLM3 and Huh7 cells was evaluated with CCK-8 (b), colony formation (c), flow cytometry (d), wound healing (e), or transwell assays (f). g, h Protein levels of Cyclin D1, MMP2, and MMP9 in HCCLM3 and Huh7 cells were examined via western blot analysis. *P < 0.05