Fig. 2

miR-485-3p can bind with SNHG6 in CC cells. a, b Subcellular fraction assay and FISH assay searched the SNHG6 location in SiHa and HeLa cells. Blue (DAPI): nuclei, Red: SNHG6. c MS2-RIP assay searched the binding ability of miRNAs to SNHG6. d Detection of miR-485-3p expression and miR-26a-5p expression in CC cells via qRT-PCR. e Binding sites of SNHG6 to miR-485-3p obtained from ENCORI were presented. f Overexpression efficiency of miR-485-3p was assessed via qRT-PCR in SiHa and HeLa cells after miR-485-3p mimics or NC mimics were transfected into above cells at 48 h. g, h Luciferase reporter assay and RNA pull down assay proved the binding of SNHG6 to miR-485-3p in SiHa and HeLa cells. **p < 0.01