Fig. 3

STYX is the downstream target of miR-485-3p and functions as an oncogene in CC cells. a Target genes of miR-485-3p were explored via ENCORI website in accordance with the intersection of four bioinformatics tools. b qRT-PCR analysis of mRNAs that could be affected by silenced SNHG6 or overexpressed miR-485-3p. c Expression levels of six candidate mRNAs (CREBRF, STYX, SEPT2, STK35, RHOA and TNPO1) were assessed via qRT-PCR in CC cells. d Binding site of STYX to miR-485-3p obtained from ENCORI was presented. e Luciferase reporter assay revealed the luciferase activity of STYX-WT or STYX-Mut in cells co-transfected with NC mimics or miR-485-3p mimics. f RNA pull down assay detected the enrichment of STYX in cells incubated with bio-NC, bio-miR-485-3p-WT or bio-miR-485-3p-Mut. g Inhibition efficiency of STYX was tested via qRT-PCR in SiHa and HeLa cells transfected with sh-NC or shRNA targeted to STYX. h, i Cell proliferation was identified via colony formation assay and EdU assay. j, k JC-1 assay and TUNEL assay assessed the apoptosis rate of SiHa and HeLa cells after silencing with STYX. l The sensitivity of CC cells to radiation was measured after silencing of STYX. **p < 0.01