Fig. 4

Knockdown of circPOSTN mediated-effects on proliferation and apoptosis of glioma cells could be eliminated by silencing miR-361-5p. a–j LN229 and U251 cells were transfected with si-NC, si-circPOSTN, si-circPOSTN + anti-miR-NC, or si-circPOSTN + anti-miR-361-5p. a, b The relativity expression level of miR-361-5p was analyzed with RT-qPCR assay in LN229 and U251 cells. c, d MTT assay was administrated to assess cell viability of LN229 and U251 cells after transfection. e, f The apoptosis of transfected LN229 and U251 cells was monitored by flow cytometry. g, h The western blot assay was employed to show the expression levels of Bcl-2 and Bax in LN229 and U251 cells. i, j The caspase-3 activity was examined by caspase-3 assay kit. *P < 0.05