Fig. 5

CircPOSTN silencing inhibited aerobic glycolysis of glioma cells via regulating miR-361-5p. a–l LN229 and U251 cells were transfected with si-NC, si-circPOSTN, si-circPOSTN + anti-miR-NC, or si-circPOSTN + anti-miR-361-5p. a–f The concentration of glucose and lactate, as well as cellular ATP level were detected with different kits. g, h The protein expression levels of HK2 and LDHA in LN229 and U251 cells were measured with western blot assay. i–l The enzyme activity of LDHA and ROS level were measured in transfected LN229 and U251 cells. *P < 0.05