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Fig. 6 | Cancer Cell International

Fig. 6

From: CircPOSTN/miR-361-5p/TPX2 axis regulates cell growth, apoptosis and aerobic glycolysis in glioma cells

Fig. 6

CircPOSTN regulated TPX2 expression via sponging miR-361-5p in glioma cells. a The binding sequences between miR-361-5p and TPX2, and matched mutant sites were shown. b, c Luciferase activity was determined by dual-luciferase reporter assay in LN229 and U251 cells following co-transfection with TPX2 3′UTR-WT or TPX2 3′UTR-MUT and miR-361-5p or miR-NC according to the design. dg The mRNA and protein expression levels of TPX2 were estimated using RT-qPCR and western blot assays in glioma tissues and cells, along with controls. h, i The correlation analysis between TPX2 and miR-361-5p or circPOSTN was conducted by Pearson’s correlation analysis. j, k The RT-qPCR and western blot assays were recruited to evaluate the expression levels of TPX2 level in LN229 and U251 cells transfected with miR-NC, miR-361-5p, miR-361-5p + pcDNA-NC or miR-361-5p + pcDNA-circPOSTN. *P < 0.05

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