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Fig. 2 | Cancer Cell International

Fig. 2

From: CircHIPK3/miR-876-5p/PIK3R1 axis regulates regulation proliferation, migration, invasion, and glutaminolysis in gastric cancer cells

Fig. 2

Effects of circHIPK3 silencing on proliferation, migration, invasion, and glutaminolysis in gastric cancer cells. ak HGC-27 and AGS cells were divided into two groups: si-NC and si-circHIPK3 groups. a The expression level of circHIPK3 was evaluated by RT-qPCR assay in HGC-27 and AGS cells. b, c The growth curves of HGC-27 and AGS cells were analyzed using MTT assay. d, e Transwell migration and invasion assays were performed in HGC-27 and AGS cells. fh The levels of glutamine, glutamate, and α-KG were displayed in HGC-27 and AGS cells. ik Western blot analysis was conducted to measure ASCT2 and GLS1 levels in HGC-27 and AGS cells. *P < 0.05

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