Fig. 2

Effects of circHIPK3 silencing on proliferation, migration, invasion, and glutaminolysis in gastric cancer cells. a–k HGC-27 and AGS cells were divided into two groups: si-NC and si-circHIPK3 groups. a The expression level of circHIPK3 was evaluated by RT-qPCR assay in HGC-27 and AGS cells. b, c The growth curves of HGC-27 and AGS cells were analyzed using MTT assay. d, e Transwell migration and invasion assays were performed in HGC-27 and AGS cells. f–h The levels of glutamine, glutamate, and α-KG were displayed in HGC-27 and AGS cells. i–k Western blot analysis was conducted to measure ASCT2 and GLS1 levels in HGC-27 and AGS cells. *P < 0.05