Fig. 4

Knockdown of circHIPK3-mediated effects on proliferation, migration, invasion, and glutaminolysis were overturned by depletion of miR-876-5p in gastric cancer cells. a–k HGC-27 and AGS cells were transfected with si-NC, si-circHIPK3, si-circHIPK3 + anti-miR-NC, or si-circHIPK3 + anti-miR-876-5p. a The expression level of miR-876-5p was estimated by RT-qPCR assay in HGC-27 and AGS cells. b, c MTT assay was used to detect the cell viability of HGC-27 and AGS cells. d, e Transwell assay was performed to assess migration and invasion abilities of HGC-27 and AGS cells. f–h The levels of glutamine, glutamate, and α-KG were examined in HGC-27 and AGS cells. i–k The protein expression levels of ASCT2 and GLS1 in HGC-27 and AGS cells were assessed by western blot analysis. *P < 0.05