Fig. 6

Overexpression of miR-876-5p inhibited proliferation, migration, invasion and glutaminolysis of gastric cancer cells by affecting PIK3R1. a–k HGC-27 and AGS cells were transfected with miR-NC, miR-876-5p, miR-876-5p +vector, or miR-876-5p + PIK3R1. a The protein expression level of PIK3R1 was measured by western blot assay in HGC-27 and AGS cells. b, c The cell proliferation was assessed by MTT assay in HGC-27 and AGS cells. d, e The cell migration and invasion were measured by transwell assay in HGC-27 and AGS cells. f–h Glutaminolysis of HGC-27 and AGS cells was determined by measuring the levels of glutamine, glutamate, and α-KG. i–k The expression levels of ASCT2 and GLS1 were examined by western blot analysis in HGC-27 and AGS cells. *P < 0.05