Fig. 2

TET2 inhibiting proliferation and invasion of NPC cells is independent on TET2’s catalytic activity. a TET2 expression in wild type (WT) CNE1 cells or TET2 knockout-(TET2-cas9) CNE1 cells was examined by western blotting. Cell viability (b) and cell invasive ability (c) of TET2-knockdown CNE1 cells were examined. d TET2 expression in CNE1 cells (control), CNE1 cells infected with lentivirus expressing flag-tagged TET2 (lenti-TET2), or CNE1 cells infected with flag-tagged TET2 mutant with deficiency of catalytic activity, was examined by western blotting. Cell viability (e) and cell invasive ability (f) of the indicated cells were examined. g TET2 expression in DMSO (control), TET2 inhibitor Bobcat339 (BC339, 80 μM), or dimethyloxallyl glycine (DMOG, 10 μM), treated CNE1 cells were examined. h 5hmc levels of the indicated cellular genome was detected by dot-blot. Cell viability (i) and cell invasive ability (j) of the indicated cells were examined. Data are represented as mean ± SD (n = 3; *represents P < 0.05)